high resolution microcomputed tomography micro ct scans  (Skyscan Corporation)

Journal: bioRxiv

Article Title: Transcriptomic landscape of human induced pluripotent stem cell-derived osteogenic differentiation identifies a regulatory role of KLF16

doi: 10.1101/2024.02.11.579844

Figure Lengend Snippet: a The expression of Klf16 at three human osteogenic differentiation stages. Data are shown as mean + SEM. * MSC vs. preOB, # preOB vs. OB, or + MSC vs. OB, adjusted p value < 0.001. b Analysis of Klf16 expression by RT-qPCR in MC3T3-E1 cells transduced vectors containing either stuffer sequence or Klf16 cDNA. Data are presented as the mean + SEM (n=3, unpaired t-test p < 0.01). c Osteogenic differentiation of MC3T3-E1 cells without or with overexpression of Klf16 , stained for ALP at day seven and alizarin red and von Kossa at day 14 and 21. d Length, fat mass, and lean mass of wild type (WT) and Klf16 +/- mice. e DEXA analysis of whole body (head excluded) bone mineral content (BMC), bone area (B-area), and bone mineral density (BMD) of WT and Klf16 +/- mice. f and g Representative microCT images of distal femur trabecular bone in f ( left, top view; right , side view) and cortical bone in g from WT and Klf16 +/- mice. Scale bar: 1 mm. h and i Graphs show trabecular bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), and trabecular separation (Tb.Sp) in h ; cortical bone area (Ct.Ar), cortical periosteal perimeter (Ct.Pe.Pm), and cortical endosteal perimeter (Ct.En.Pm) in i . For d , e , h , and i , data are presented using BoxPlotR as the mean + SEM (WT n = 6, Klf16 +/- n = 6, 3 males and 3 females for each group, aged 17 weeks, paired t-test, * p < 0.05, ** p <0.01).

Article Snippet: Femurs were isolated by removing attached soft tissues, fixed in 4% PFA at 4°, and imaged with microcomputed tomography (microCT) scan (Skyscan 1172a, Skyscan, Belgium) at 50 kV and 200 µA using a 0.5 mm aluminum filter and an image voxel size of 4.5 µm isotropic.

Techniques: Expressing, Quantitative RT-PCR, Sequencing, Over Expression, Staining

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Local Sustained Delivery of Anti–IL-17A Antibodies Limits Inflammatory Bone Loss in Murine Experimental Periodontitis

doi: 10.4049/jimmunol.2001432

Figure Lengend Snippet: microCT evaluation of alveolar bone loss in mice. MP were injected into gingival tissue surrounding the ligated tooth on either day 0 or day 2 after ligature placement. Representative two-dimensional microCT images from sagittal and transaxial slices of mice hemimaxilla as follows: healthy group, untreated group, and groups treated with anti–IL-17A MP at day 0 and day 2. Quantification of alveolar bone loss represented by the linear bone loss between the CEJ and ABC (dashed lines) along the interdental (A), buccal (B), and palatal (C) sides. Values (mean ± SD) obtained from 5–6 animals per group. The p values were determined by one-way ANOVA, followed by a Tukey multiple comparisons test. *p < 0.05, **p < 0.005, ***p <0.0005, ****p < 0.0001.

Article Snippet: Alveolar bone loss quantification To quantify alveolar bone loss, the fixed mice maxillae, were transferred to 70% ethanol for microcomputed tomography (microCT) scanning using a viva CT 40 microCT system (SCANCO Medical, Brüttisellen, Switzerland) at a resolution of 10.5 μm and 55 μA kVp.

Techniques: Injection

Journal: Journal of Orthopaedic Translation

Article Title: Investigation of bioeffects of G protein-coupled receptor 1 on bone turnover in male mice

doi: 10.1016/j.jot.2017.05.001

Figure Lengend Snippet: GPR1 −/− deficiency male mice have much lower bone mineral density. (A) MicroCT images of secondary spongy bone of the metaphysis of distal femora from GPR1 −/− and WT control mice. (B) The quantitative microCT bone parameters BMD, BV/TV, Tb.Th, Tb.N, DA, SMI, Tb.Pf, and Tb.Sp. Statistical significance was assessed with two-tailed Student t test; ** p < 0.01 versus WT, *** p < 0.001 versus WT, N WT = 15, N GPR1 −/− = 6. BMD = bone mineral density; BV/TV = ratio of bone volume to tissue volume; DA = degree anisotropy; GPR1 = G protein-coupled receptor 1; microCT = microcomputed tomography; SMI = structure model index; Tb.N = trabecular number; Tb.Pf = trabecular pattern factor; Tb.Sp = trabecular separation; Tb.Th = trabecular thickness; WT = wild type.

Article Snippet: The femora of 12-month-old WT ( N = 15) and GPR1 −/− male mice ( N = 6) were microcomputed tomography (microCT) scanned (SkyScan 1176; Bruker, Kontich, Belgium) after dissection of the animals that were euthanised by CO 2 inhalation.

Techniques: Control, Two Tailed Test, Tomography